Use of Translation Blocking Morpholinos for Gene Knockdown in Giardia lamblia.
Methods Mol Biol
; 1565: 123-140, 2017.
Article
em En
| MEDLINE
| ID: mdl-28364239
ABSTRACT
Giardia lamblia, a major parasite, is an emerging model organism due to its compact genomic arrangement and composition. The most popular reverse genetic technique, RNAi, is ineffective in Giardia. In contrast, protein depletion by translation blocking morpholinos is suitable for most gene targets and provides up to 80% depletion of the target protein. The method is fast, reliable, and specific. After antisense morpholino oligomer delivery into Giardia trophozoites by electroporation, the cells can be used for many subsequent analyses 8-48 h after treatment. In this chapter, suitable gene tags, plasmids, and techniques necessary for proper morpholino targeting are described.
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Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
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Proteínas de Protozoários
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Giardia lamblia
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Técnicas de Silenciamento de Genes
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Morfolinos
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2017
Tipo de documento:
Article