Protective effect of thymoquinone against cyclophosphamide-induced genotoxic damage in human lymphocytes.
Bratisl Lek Listy
; 118(4): 208-211, 2017.
Article
em En
| MEDLINE
| ID: mdl-28471230
ABSTRACT
OBJECTIVE:
Protective effect of thymoquinone (TQ) against the cytotoxic and genotoxic effects of cyclophosphamide (CP) was assessed in human peripheral blood lymphocyte culture.METHODS:
Mitotic indices were determined as endpoints of cytotoxicity, while sister chromatid exchanges (SCE) served as endpoints of genotoxicity. Firstly, the genotoxic effect of 0.16 µg/ml of CP was tested and CP was detected as genotoxic. In the second set, CP group was treated with 20 µM and 40 µM TQ.RESULTS:
TQ reduced the SCE frequencies, suggesting its protective action on human lymphocytes in vitro against the CP induced genotoxic damage.CONCLUSIONS:
Our results suggest that TQ produces a protective mechanism against CP-induced genetic damage, and suggest a role of DNA strand breaks in the genotoxicity (Tab. 1, Fig. 1, Ref. 19).Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Troca de Cromátide Irmã
/
Benzoquinonas
/
Ciclofosfamida
Limite:
Humans
Idioma:
En
Revista:
Bratisl Lek Listy
Ano de publicação:
2017
Tipo de documento:
Article