Analysis of RBC-microparticles in stored whole blood bags - a promising marker to detect blood doping in sports?
Drug Test Anal
; 9(11-12): 1794-1798, 2017 Nov.
Article
em En
| MEDLINE
| ID: mdl-28474406
ABSTRACT
Blood doping in sports is prohibited by the World Anti-Doping Agency (WADA). To find a possible biomarker for the detection of blood doping, we investigated the changes in blood stored in CPDA-1 blood bags of eight healthy subjects who donated one unit of blood. Aliquots were taken on days 0, 14, and 35. Platelet-free plasma was prepared and stored at -80°C until analysis on a flow cytometer dedicated for the analysis of microparticles (MPs). Changes in the number of red blood cell (RBC) -MPs were highly significant (p < 0.0001) with a mean of 219 (10^3/µL) on day 0 changing to 23 120 (10^3/µL) on day 14 and 29 310 (10^3/µL) on day 35. We conclude that RBC-MPs seem to be a promising biomarker for doping control but confirmation by a transfusion study is necessary.
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Coleções:
01-internacional
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MEDLINE
Assunto principal:
Fosfatos
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Adenina
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Biomarcadores
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Citratos
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Dopagem Esportivo
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Eritrócitos
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Glucose
Idioma:
En
Revista:
Drug Test Anal
Ano de publicação:
2017
Tipo de documento:
Article