Involvement of cystathionase in the formation of alkane-thiols from corresponding cysteine conjugates.
Xenobiotica
; 18(9): 1029-37, 1988 Sep.
Article
em En
| MEDLINE
| ID: mdl-2852420
ABSTRACT
1. Rat liver cystathionase [EC.4.4.1.1] mediated a cleavage reaction of the C-S bond of S-alkyl-L-cysteine conjugates to give equimolar amounts of corresponding thiols, ammonia, and pyruvic acid. Neither S-aryl- nor S-aralkyl-L-cysteine conjugates were acceptable substrates. 2. The Km value for S-(tert-butyl)-L-cysteine was 0.3 mM at pH 8.5 in Tris-HCl buffer. 3. The S-alkyl-L-cysteine lyase activity of cystathionase was inhibited with carbonyl reagents and dithiothreitol. 4. The present finding that cystathionase has activity towards cysteine conjugates may provide some insights into the role of this enzyme in the metabolism of xenobiotics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cistationina gama-Liase
/
Cisteína
/
Fígado
/
Liases
Limite:
Animals
Idioma:
En
Revista:
Xenobiotica
Ano de publicação:
1988
Tipo de documento:
Article