Immuno-PCR, a new technique for the serodiagnosis of tuberculosis.
J Microbiol Methods
; 139: 218-229, 2017 08.
Article
em En
| MEDLINE
| ID: mdl-28527886
ABSTRACT
Rapid and accurate diagnosis of tuberculosis (TB) is essential to control the disease. The conventional microbiological tests have limitations and there is an urgent need to devise a simple, rapid and reliable point-of-care (POC) test. The failure of TB diagnostic tests based on antibody detection due to inconsistent and imprecise results has stimulated renewed interest in the development of rapid antigen detection methods. However, the World Health Organization (WHO) has emphasized to continue research for designing new antibody-based detection tests with improved accuracy. Immuno-polymerase chain reaction (I-PCR) combines the simplicity and versatility of enzyme-linked immunosorbent assay (ELISA) with the exponential amplification capacity and sensitivity of PCR thus leading to several-fold increase in sensitivity in comparison to analogous ELISA. In this review, we have described the serodiagnostic potential of I-PCR assays for an early diagnosis of TB based on the detection of potential mycobacterial antigens and circulating antibodies in body fluids of TB patients.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
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Tuberculose Pulmonar
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Ensaio de Imunoadsorção Enzimática
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Testes Sorológicos
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Reação em Cadeia da Polimerase
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2017
Tipo de documento:
Article