Artificial liver support in pigs with acetaminophen-induced acute liver failure.
World J Gastroenterol
; 23(18): 3262-3268, 2017 May 14.
Article
em En
| MEDLINE
| ID: mdl-28566885
ABSTRACT
AIM:
To establish a reversible porcine model of acute liver failure (ALF) and treat it with an artificial liver system.METHODS:
Sixteen pigs weighing 30-35 kg were chosen and administered with acetaminophen (APAP) to induce ALF. ALF pigs were then randomly assigned to either an experimental group (n = 11), in which a treatment procedure was performed, or a control group (n = 5). Treatment was started 20 h after APAP administration and continued for 8 h. Clinical manifestations of all animals, including liver and kidney functions, serum biochemical parameters and survival times were analyzed.RESULTS:
Twenty hours after APAP administration, the levels of serum aspartate aminotransferase, total bilirubin, creatinine and ammonia were significantly increased, while albumin levels were decreased (P < 0.05). Prothrombin time was found to be extended with progression of ALF. After continuous treatment for 8 h (at 28 h), aspartate aminotransferase, total bilirubin, creatinine, and ammonia showed a decrease in comparison with the control group (P < 0.05). A cross-section of livers revealed signs of vacuolar degeneration, nuclear fragmentation and dissolution. Concerning survival, porcine models in the treatment group survived for longer times with artificial liver system treatment (P < 0.05).CONCLUSION:
This model is reproducible and allows for quantitative evaluation of new liver systems, such as a bioartificial liver. The artificial liver system (ZHJ-3) is safe and effective for the APAP-induced porcine ALF model.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Falência Hepática Aguda
/
Fígado Artificial
/
Hepatócitos
/
Fígado
/
Acetaminofen
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
World J Gastroenterol
Ano de publicação:
2017
Tipo de documento:
Article