Protective effects of asiatic acid in a spontaneous type 2 diabetic mouse model.
Mol Med Rep
; 16(2): 1333-1339, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28586016
Asiatic acid (AA) has been demonstrated to exhibit anti-diabetic activity. However, the mechanisms and underlying signaling pathways remain to be elucidated. The present study was performed to confirm the protective effect of AA and demonstrate its ability to regulate the phosphatidylinositol 3kinase (PI3K)/protein kinase B (AKT)/glycogen synthase kinase3ß (GSK3ß) signaling pathway in db/db mice. Db/db mice fed on a highfat diet were used to model diabetes mellitus. Modeled mice were divided randomly into the model control, pioglitazone hydrochloride tablet (PH) and AA groups. Agematched C57 BL/6J mice served as normal controls. Lipid and glucose levels, and glycogen synthesis rates were assessed following treatment. Pathological changes were detected using hematoxylin and eosin staining. Expression of the PI3K/AKT/GSK3ß signaling pathway at the mRNA level was measured using quantitative polymerase chain reaction analysis. The model control group revealed typical characteristics of obesity and diabetes, including high glucose and lipid levels, and decreased glycogen synthesis. Four weeks of treatment with AA or PH ameliorated these abnormalities. AA and PH treatments mitigated the upregulation of PI3K, AKT, insulin receptor, and insulin receptor substrate1 mRNA expression in modeled mice. Furthermore, AA and PH treatments decreased GSK3ß and glucose6phosphatase mRNA expression compared with the normal control group. The results of the present study confirmed that AA possesses antidiabetic activity in db/db mice. The PI3K/AKT/GSK3ß signaling pathway may mediate this protective effect.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diabetes Mellitus Tipo 2
/
Triterpenos Pentacíclicos
/
Hipoglicemiantes
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2017
Tipo de documento:
Article