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Early protection to stress mediated by CDK-dependent PI3,5P2 signaling from the vacuole/lysosome.
Jin, Natsuko; Jin, Yui; Weisman, Lois S.
Afiliação
  • Jin N; Life Sciences Institute, University of Michigan, Ann Arbor, MI.
  • Jin Y; Life Sciences Institute, University of Michigan, Ann Arbor, MI.
  • Weisman LS; Life Sciences Institute, University of Michigan, Ann Arbor, MI lweisman@umich.edu.
J Cell Biol ; 216(7): 2075-2090, 2017 07 03.
Article em En | MEDLINE | ID: mdl-28637746
Adaptation to environmental stress is critical for cell survival. Adaptation generally occurs via changes in transcription and translation. However, there is a time lag before changes in gene expression, which suggests that more rapid mechanisms likely exist. In this study, we show that in yeast, the cyclin-dependent kinase Pho85/CDK5 provides protection against hyperosmotic stress and acts before long-term adaptation provided by Hog1. This protection requires the vacuolar/endolysosomal signaling lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells. Moreover, this regulation is particularly crucial in yeast for the stress-induced transient elevation of PI3,5P2 Our study reveals a rapid protection mechanism regulated by Pho85/CDK5 via signaling from the vacuole/lysosome, which is distinct temporally and spatially from the previously discovered long-term adaptation Hog1 pathway, which signals from the nucleus.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pressão Osmótica / Endossomos / Saccharomyces cerevisiae / Vacúolos / Sistemas do Segundo Mensageiro / Fosfotransferases (Aceptor do Grupo Álcool) / Fosfatos de Fosfatidilinositol / Quinases Ciclina-Dependentes / Proteínas de Saccharomyces cerevisiae / Lisossomos Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pressão Osmótica / Endossomos / Saccharomyces cerevisiae / Vacúolos / Sistemas do Segundo Mensageiro / Fosfotransferases (Aceptor do Grupo Álcool) / Fosfatos de Fosfatidilinositol / Quinases Ciclina-Dependentes / Proteínas de Saccharomyces cerevisiae / Lisossomos Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2017 Tipo de documento: Article