A new radiochemical method for determination of pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase.
Anal Biochem
; 148(1): 182-9, 1985 Jul.
Article
em En
| MEDLINE
| ID: mdl-2864000
ABSTRACT
A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-14C]pyruvate and [1-14C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically formed acetyl-coenzyme A. [1-14C]Acetyl-DTE is easily extracted from the incubation mixture by organic solvents and separated from the unreacted labeled substrates.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetato-CoA Ligase
/
Complexo Piruvato Desidrogenase
/
Coenzima A Ligases
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1985
Tipo de documento:
Article