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Sample-to-answer acoustic detection of DNA in complex samples.
Papadakis, George; Palladino, Pasquale; Chronaki, Dimitra; Tsortos, Achilleas; Gizeli, Electra.
Afiliação
  • Papadakis G; Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. gizeli@imbb.forth.gr.
  • Palladino P; Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. gizeli@imbb.forth.gr.
  • Chronaki D; Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. gizeli@imbb.forth.gr and Dept. of Biology, Univ. of Crete, Vassilika Vouton, 71409, Greece.
  • Tsortos A; Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. gizeli@imbb.forth.gr.
  • Gizeli E; Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str, Heraklion, 70013, Greece. gizeli@imbb.forth.gr and Dept. of Biology, Univ. of Crete, Vassilika Vouton, 71409, Greece.
Chem Commun (Camb) ; 53(57): 8058-8061, 2017 Jul 13.
Article em En | MEDLINE | ID: mdl-28671702
ABSTRACT
The present study demonstrates the sensitive and label-free acoustic detection of dsDNA amplicons produced from whole Salmonella Thyphimurium cells without employing any DNA extraction and/or purification step, in the presence of the lysed bacterial cells and in a hybridization-free assay. A sample-to-answer assay is also shown during DNA detection directly in milk.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Salmonella / DNA Bacteriano / Leite Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Chem Commun (Camb) Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Salmonella / DNA Bacteriano / Leite Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Chem Commun (Camb) Ano de publicação: 2017 Tipo de documento: Article