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Serotonin induces ecdysteroidogenesis and methyl farnesoate synthesis in the mud crab, Scylla serrata.
Girish, B P; Swetha, C H; Reddy, P Sreenivasula.
Afiliação
  • Girish BP; Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, India; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Swetha CH; Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, India; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
  • Reddy PS; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India. Electronic address: psreddy1955@gmail.com.
Biochem Biophys Res Commun ; 490(4): 1340-1345, 2017 09 02.
Article em En | MEDLINE | ID: mdl-28690150
ABSTRACT
In the current study, we have examined the role of serotonin in regulating the levels of methyl farnesoate and ecdysteroids in the giant mud crab Scylla serrata and validated that serotonin indeed is a reproductive hormone. Administration of serotonin elevated circulatory levels of methyl farnesoate and ecdysteroids in crabs. Since methyl farnesoate and ecdysteroid act through retinoid X receptor (RXR) and ecdysteroid receptor (EcR) respectively and these receptors are involved in the regulation of reproduction in crustaceans, we have determined the mRNA levels of RXR and EcR in hepatopancreas and ovary after serotonin administration. The expression levels of both RXR and EcR increased significantly in the hepatopancreas and ovary of serotonin injected crabs when compared to the controls. In vitro organ culture studies revealed that incubation of Y-orgas and mandibular organ explants in the presence of serotonin resulted in a significant increase in the secretion of ecdysteroids by Y-organs, but without alterations in MF synthesis in mandibular organs. From the above studies it is evident that serotonin stimulates Y organs resulting in increased ecdysteroidogenesis. Though the circulatory levels methyl farnesoate elevated after serotonin administration, organ culture studies revealed serotonin mediated methyl farnesaote synthesis is indirect probably by inhibiting release of mandibular organ inhibiting hormone from eyestalks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Esteroides / Serotonina / Braquiúros / Ecdisteroides / Ácidos Graxos Insaturados / Proteínas de Artrópodes Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Esteroides / Serotonina / Braquiúros / Ecdisteroides / Ácidos Graxos Insaturados / Proteínas de Artrópodes Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2017 Tipo de documento: Article