Differential neuronal and glial behavior on flat and micro patterned chitosan films.
Colloids Surf B Biointerfaces
; 158: 569-577, 2017 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-28750339
ABSTRACT
Chitosan is a biodegradable natural polysaccharide that has been widely studied for regenerative purposes in the central nervous system. In this study we assessed the in vitro glial and neuronal cells response to chitosan either flat or patterned with grooves in the micrometric range. Chitosan demonstrated to be a good substrate for the attachment and growth of both neurons and glial cells. Chitosan micropatterns promoted glial cell maturation, suggesting astroglial activation. Nevertheless, those mature/reactive glial cells were permissive for axonal growth. Axons aligned and organized along the patterned grooves and the size of the linear topographic patterns is also affecting neurite and cell response. Patterns with 10µm width induced fasciculation of axons, which can be useful for CNS tissue engineering substrates when precise orientation of the axonal outgrowth is desired.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neuroglia
/
Quitosana
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Neurônios
Limite:
Animals
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Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Ano de publicação:
2017
Tipo de documento:
Article