In Vitro Cryopreservation of Date Palm Caulogenic Meristems.
Methods Mol Biol
; 1638: 39-48, 2017.
Article
em En
| MEDLINE
| ID: mdl-28755212
ABSTRACT
Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant. Regeneration rates obtained with standard vitrification, encapsulation-vitrification, and droplet-vitrification protocols can reach 30, 40, and 70%, respectively. All regenerated plants from non-cryopreserved or cryopreserved explants don't show morphological variation by maintaining genetic integrity without adverse effect of cryogenic treatment. Cryopreservation of date palm vitro-cultures enables commercial tissue culture laboratories to move to large-scale propagation from cryopreserved cell lines producing true-to-type plants after clonal field-testing trials. When comparing the cost of cryostorage and in-field conservation of date palm cultivars, tissue cryopreservation is the most cost-effective. Moreover, many of the risks linked to field conservation like erosion due to climatic, edaphic, and phytopathologic constraints are circumvented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Phoeniceae
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2017
Tipo de documento:
Article