DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation.
PLoS One
; 12(8): e0182283, 2017.
Article
em En
| MEDLINE
| ID: mdl-28763495
ABSTRACT
We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. These results underscore the utility of DNA barcoding to aid specimen identification in diverse biological hotspots, areas that require urgent action regarding taxonomic surveys and biodiversity conservation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Bases de Dados Genéticas
/
Biodiversidade
/
Código de Barras de DNA Taxonômico
/
Odonatos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
PLoS One
Ano de publicação:
2017
Tipo de documento:
Article