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Capillary-Inserted Rotor Design for HRµMASNMR-Based Metabolomics on Mass-Limited Neurospheres.
Duong, Nghia Tuan; Yamato, Masanori; Nakano, Masayuki; Kume, Satoshi; Tamura, Yasuhisa; Kataoka, Yosky; Wong, Alan; Nishiyama, Yusuke.
Afiliação
  • Duong NT; Advanced Solid-State NMR Unit, RIKEN CLST-JEOL Collaboration Center, RIKEN, Yokohama, Kanagawa 230-0045, Japan. nghiatuan.duong@riken.jp.
  • Yamato M; Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, RIKEN, Kobe, Hyogo 650-0047, Japan. yamatomasa@riken.jp.
  • Nakano M; Cellular Function Imaging Team, RIKEN Center for Life Science Technologies, Kobe, Hyogo 650-0047, Japan. yamatomasa@riken.jp.
  • Kume S; Cellular Function Imaging Team, RIKEN Center for Life Science Technologies, Kobe, Hyogo 650-0047, Japan. cannakano@gmail.com.
  • Tamura Y; Department of Physiology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka 545-8585, Japan. cannakano@gmail.com.
  • Kataoka Y; Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, RIKEN, Kobe, Hyogo 650-0047, Japan. satoshi.kume@riken.jp.
  • Wong A; Cellular Function Imaging Team, RIKEN Center for Life Science Technologies, Kobe, Hyogo 650-0047, Japan. satoshi.kume@riken.jp.
  • Nishiyama Y; Multi-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, RIKEN, Kobe, Hyogo 650-0047, Japan. tamuray@riken.jp.
Molecules ; 22(8)2017 Aug 03.
Article em En | MEDLINE | ID: mdl-28771206
ABSTRACT
Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique and has been widely used in metabolomics. However, the intrinsic low sensitivity of NMR prevents its applications to systems with limited sample availabilities. In this study, a new experimental approach is presented to analyze mass-scarce samples in limited volumes of less than 300 nL with simple handling. The sample is loaded into the glass capillary, and this capillary is then inserted into a Kel-F rotor. The experimental performance of the capillary-inserted rotor (capillary-insert) is investigated on an isotropic solution of sucrose by the use of a high-resolution micro-sized magic angle spinning (HRµMAS) probe. The acquired NMR signal's sensitivity to a given sample amount is comparable or even higher in comparison to that recorded by the standard solution NMR probe. More importantly, this capillary-insert coupled with the HRµMAS probe allows in-depth studies of heterogeneous samples as the MAS removes the line broadening caused by the heterogeneity. The NMR analyses of mass-limited cultured neurospheres have been demonstrated, resulting in high quality spectra where numerous metabolites are unambiguously identified.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância Magnética / Metabolômica Idioma: En Revista: Molecules Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância Magnética / Metabolômica Idioma: En Revista: Molecules Ano de publicação: 2017 Tipo de documento: Article