Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli.

Edri, Ilan; Goldenberg, Michal; Lisnyansky, Michal; Strulovich, Roi; Newman, Hadas; Loewenstein, Anat; Khananshvili, Daniel; Giladi, Moshe; Haitin, Yoni

Edri, Ilan; Goldenberg, Michal; Lisnyansky, Michal; Strulovich, Roi; Newman, Hadas; Loewenstein, Anat; Khananshvili, Daniel; Giladi, Moshe; Haitin, Yoni.

Afiliação

Edri I; Sackler Faculty of Medicine, Tel Aviv University.
Goldenberg M; Sackler Faculty of Medicine, Tel Aviv University.
Lisnyansky M; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University.
Strulovich R; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University.
Newman H; Sackler Faculty of Medicine, Tel Aviv University; Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University.
Loewenstein A; Sackler Faculty of Medicine, Tel Aviv University; Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University.
Khananshvili D; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University.
Giladi M; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University; Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University; moshegil@post.tau.ac.il.
Haitin Y; Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University; yhaitin@post.tau.ac.il.

J Vis Exp ; (126)2017 08 03.

Article em En | MEDLINE | ID: mdl-28809830

Assuntos

Alquil e Aril Transferases/isolamento & purificação; Escherichia coli/genética; Engenharia de Proteínas/métodos; Proteínas Recombinantes de Fusão/isolamento & purificação; Alquil e Aril Transferases/genética; Alquil e Aril Transferases/metabolismo; Cromatografia em Gel/métodos; Clonagem Molecular/métodos; Códon; Escherichia coli/metabolismo; Humanos; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/metabolismo; Transferases/genética; Transferases/metabolismo

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Engenharia de Proteínas / Alquil e Aril Transferases / Escherichia coli Limite: Humans Idioma: En Revista: J Vis Exp Ano de publicação: 2017 Tipo de documento: Article

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