A clarifying method that improves imaging of Aplysia ganglia.
Sheng Li Xue Bao
; 69(4): 461-466, 2017 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-28825105
ABSTRACT
Improvements in the imaging of neural circuits are essential for studies of network function in both invertebrates and vertebrates. Therefore, CLARITY, a new imaging enhancement technique developed for mouse brains has attracted broad interest from researchers working on other species. We studied the potential of a modified version of CLARITY to enhance the imaging of ganglia in an invertebrate Aplysia. For example, we have modified the hydrogel solution and designed a small container for the Aplysia ganglia. The ganglia were first processed for immunohistochemistry, and then for CLARITY. We examined the compatibility of these techniques and the extent to which the imaging of fluorescence improved using confocal microscopy. We found that CLARITY did indeed enhance the imaging of CP2 immunopositive neurons in Aplysia ganglia. For example, it improved visualization of small, weak immunoreactive neurons deep in the ganglia. Our modifications of CLARITY make this new method suitable for future use in Aplysia experiments. Furthermore, our techniques are likely to facilitate imaging in other invertebrate ganglia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aplysia
/
Aumento da Imagem
/
Gânglios dos Invertebrados
Limite:
Animals
Idioma:
En
Revista:
Sheng Li Xue Bao
Ano de publicação:
2017
Tipo de documento:
Article