A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS.
Clin Chim Acta
; 473: 116-123, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28842174
ABSTRACT
BACKGROUND:
Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3.METHODS:
Sample preparation was based on precipitation and centrifugation of 100µL of patient serum, followed by injection into the LC-MS/MS system. Samples from Vitamin D Standardization Program (n=80) and patient samples (n=281) have been compared with a reference LC-MS/MS method. For the analytical validation NIST and Labquality quality control materials were used.RESULTS:
Mean intra-assay and inter-assay imprecision were <6.0 and 6.4% and mean recoveries were within 95-104%. LOQ's were 0.5µg/L for 24,25(OH)2D3, 1.1µg/L for 25(OH)D3 and epi-25(OH)D3 and 1.7µg/L for 25(OH)D2. A 3% bias obtained between the proposed and the reference method satisfies Vitamin D Standardization Program recommendations.CONCLUSIONS:
We present a rapid, easy, reliable and cost-effective method completely adequate for routine testing, which permits the measurement of the ratio of 24,25-dihydroxyvitamin D to 25-hydroxyvitamin D, Vitamin D Metabolite Ratio (VMR), in serum samples.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vitamina D
/
Análise Química do Sangue
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
2017
Tipo de documento:
Article