Pediatric reference data on activity of urinary N-acetyl-ß-D-hexosaminidase and its isoenzymes.
Adv Med Sci
; 63(1): 94-99, 2018 Mar.
Article
em En
| MEDLINE
| ID: mdl-28846871
ABSTRACT
PURPOSE:
The objective of the study was to establish age - dependent values of the urinary lysosomal exoglycosidases activities N-acetyl-ß-D-hexosaminidase (HEX) and its isoenzyme A (HEX A) as well as isoenzyme B (HEX B) in healthy children and adolescents. MATERIAL ANDMETHODS:
The study was performed using a random sample of 203 healthy children and adolescents (girls=99, boys=104), aged six months to 17.9 years. The activities of HEX, HEX A and HEX B were determined by a colorimetric method. The activities of the urinary HEX and its isoenzymes were expressed in pKat/µg of creatinine (pKat/µg Cr).RESULTS:
Median concentrations of urinary HEX, and its HEX A, HEX B isoenzymes in particular age groups were analyzed using ANOVA. Urinary HEX, HEX A and HEX B activities (pKat/µg Cr) were the highest in children below 3 years, in comparison to remaining age groups. There were statistically significant negative correlations between urinary HEX, HEX A as well as HEX B and age (r=-0.24, p<0.001 (HEX); r=-0.20, p<0.01 (HEX A); r=-0.26, p<0.001 (HEX B), respectively. We constructed the reference values for urinary activity of HEX, HEX A and HEX B (pKat/µg Cr) in centiles according to age, in three-year intervals.CONCLUSIONS:
Reported data present, for the first time, reference values for urinary activities of HEX and its isoenzymes HEX A and HEX B in children and adolescent.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-N-Acetil-Hexosaminidases
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Isoenzimas
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Idioma:
En
Revista:
Adv Med Sci
Ano de publicação:
2018
Tipo de documento:
Article