Enhanced Skin Regeneration Using a Novel Amniotic-derived Tissue Graft.
Wounds
; 29(9): 277-285, 2017 Sep.
Article
em En
| MEDLINE
| ID: mdl-28933697
ABSTRACT
BACKGROUND:
Chronic and recalcitrant wounds present a significant therapeutic challenge. Amniotic tissues contain many regenerative cytokines, growth factors, and extracellular matrix molecules including proteoglycans, hyaluronic acid, and collagens I, III, and IV. Dehydrated amnion/chorion grafts are currently used to treat a variety of wounds such as diabetic foot ulcers and burns.OBJECTIVE:
The investigators hypothesized that processing methodologies, dehydration, and hypothermic processing and storage of amniotic tissues would affect overall quality of wound healing; they compared dehydrated amnion/chorion (dHACM) grafts to a novel hypothermically stored amniotic membrane (HSAM) graft in a full-thickness rat wound model. MATERIALS ANDMETHODS:
Sprague-Dawley rats were anesthetized and prepped for surgery; four 1.5-cm diameter full-thickness wounds were created and treated with either (1) dHACM, (2) dHACM meshed, (3) HSAM, or (4) wound left ungrafted (sham). After 9 or 21 days, wounds and surrounding areas were collected and stained with hematoxylin and eosin. Blinded quantitative analysis of quality of wound healing was completed by evaluating hair follicle/gland formation, dense/scar-like matrix, and basket-weave matrix.RESULTS:
At varying time points following placement of the grafts into full-thickness defects, the authors found that all amniotic-derived tissue grafts appeared to stimulate improved healing over sham wounds, evidenced by more normal-appearing dermal matrix architecture, epidermal structure, and maturity. In addition, the HSAM grafts promoted greater tissue regeneration than the dHACM meshed grafts, as measured by the presence of basket-weave collagen matrix and formation of follicles and glands.CONCLUSIONS:
In sum, this study builds on the amassing literature supporting amniotic tissues for wound repair and demonstrates the importance of tissue processing on the quality of wound healing.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Cicatrização
/
Ferimentos e Lesões
/
Curativos Biológicos
/
Córion
/
Transplantes
/
Âmnio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Wounds
Ano de publicação:
2017
Tipo de documento:
Article