CLOCK stabilizes CYCLE to initiate clock function in Drosophila.
Proc Natl Acad Sci U S A
; 114(41): 10972-10977, 2017 10 10.
Article
em En
| MEDLINE
| ID: mdl-28973907
ABSTRACT
The Drosophila circadian clock keeps time via transcriptional feedback loops. These feedback loops are initiated by CLOCK-CYCLE (CLK-CYC) heterodimers, which activate transcription of genes encoding the feedback repressors PERIOD and TIMELESS. Circadian clocks normally operate in â¼150 brain pacemaker neurons and in many peripheral tissues in the head and body, but can also be induced by expressing CLK in nonclock cells. These ectopic clocks also require cyc, yet CYC expression is restricted to canonical clock cells despite evidence that cyc mRNA is widely expressed. Here we show that CLK binds to and stabilizes CYC in cell culture and in nonclock cells in vivo. Ectopic clocks also require the blue light photoreceptor CRYPTOCHROME (CRY), which is required for both light entrainment and clock function in peripheral tissues. These experiments define the genetic architecture required to initiate circadian clock function in Drosophila, reveal mechanisms governing circadian activator stability that are conserved in perhaps all eukaryotes, and suggest that Clk, cyc, and cry expression is sufficient to drive clock expression in naive cells.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Animais Geneticamente Modificados
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Proteínas de Drosophila
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Drosophila melanogaster
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Proteínas CLOCK
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Fatores de Transcrição ARNTL
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Criptocromos
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Relógios Circadianos
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2017
Tipo de documento:
Article