Multiplex Reverse Transcription-PCR for Simultaneous Surveillance of Influenza A and B Viruses.
J Clin Microbiol
; 55(12): 3492-3501, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28978683
ABSTRACT
Influenza A and B viruses are the causative agents of annual influenza epidemics that can be severe, and influenza A viruses intermittently cause pandemics. Sequence information from influenza virus genomes is instrumental in determining mechanisms underpinning antigenic evolution and antiviral resistance. However, due to sequence diversity and the dynamics of influenza virus evolution, rapid and high-throughput sequencing of influenza viruses remains a challenge. We developed a single-reaction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal influenza A and B viruses for next-generation sequencing, regardless of viral type, subtype, or lineage. Herein, we demonstrate that the strategy is highly sensitive and robust. The strategy was validated on thousands of seasonal influenza A and B virus-positive specimens using multiple next-generation sequencing platforms.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Vírus da Influenza B
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Influenza Humana
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2017
Tipo de documento:
Article