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Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System.
Buczek, Magdalena E; Reeder, Stephen P; Regad, Tarik.
Afiliação
  • Buczek ME; The John van Geest Cancer Research Centre, Nottingham Trent University, Clifton lane, Nottingham, NG11 8NS, UK.
  • Reeder SP; The John van Geest Cancer Research Centre, Nottingham Trent University, Clifton lane, Nottingham, NG11 8NS, UK.
  • Regad T; The John van Geest Cancer Research Centre, Nottingham Trent University, Clifton lane, Nottingham, NG11 8NS, UK. tarik.regad@ntu.ac.uk.
Methods Mol Biol ; 1692: 139-148, 2018.
Article em En | MEDLINE | ID: mdl-28986894
ABSTRACT
Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or immunotherapeutic assays. Here, we present a method of identification, isolation of Prostate Cancer Stem Cells (PCSCs) from the DU145 prostate cancer cell line using the NANOG-GFP expression system.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Células-Tronco Neoplásicas / Proteínas de Fluorescência Verde / Proteína Homeobox Nanog Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans / Male Idioma: En Revista: Methods Mol Biol Ano de publicação: 2018 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Próstata / Células-Tronco Neoplásicas / Proteínas de Fluorescência Verde / Proteína Homeobox Nanog Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans / Male Idioma: En Revista: Methods Mol Biol Ano de publicação: 2018 Tipo de documento: Article