Arctiin regulates collagen type 1α chain 1 mRNA expression in human dermal fibroblasts via the miR­378b­SIRT6 axis.

ABSTRACT

Arctiin, a lignin isolated from Arctium lappa, exhibits a variety of biological effects, including anti­viral, anti­inflammatory, and anti­proliferative actions, in mammalian cells. In a previous study, arctiin was demonstrated to induce procollagen type I synthesis and exhibited protective effects against ultraviolet B (UVB) radiation in normal human dermal fibroblasts (nHDFs). However, the underlying molecular mechanism of arctiin­mediated collagen synthesis remains unknown. In the present study, the mechanism for increased expression of collagen type 1α 1 chain (COL1A1) mRNA in arctiin­induced nHDFs was identified. The expression of microRNA­378b (miR­378b), downregulated by arctiin, was correlated with the expression of sirtuin­6 (SIRT6) mRNA, a regulator of COL1A1 mRNA. Furthermore, it was revealed that arctiin protected the UVB radiation­mediated decrease in COL1A1 mRNA expression, through the miR­378b/SIRT6 signaling pathway. In conclusion, these results suggest that arctiin regulates COL1A1 through the miR­378b­SIRT6 axis.