[Automatic morphometric analysis as a method for determining the level of extracellular matrix components and for quantifying nuclear antigens]. / Avtomatizirovannyi morfometricheskii analiz kak metod opredeleniia soderzhaniia komponentov vnekletochnogo matriksa i kolichestvennoi otsenki iadernykh antigenov.
Arkh Patol
; 79(5): 49-56, 2017.
Article
em Ru
| MEDLINE
| ID: mdl-29027530
ABSTRACT
Automated image analysis methods are highly important for biotechnology research. The authors developed and tested a program for the morphometric analysis of photomicrographs of the sections processed using the standard immunohistochemical examination protocols. The color deconvolution method used in the algorithm was proven to be effective in mapping the distribution of DAB chromogen in the sample containing multiple dyes. The experiment demonstrated that the level of extracellular matrix proteins could be comparatively quantified in different groups of samples. The effective methods for the quantitative analysis of the Ki-67 labelling index were also tested using the same algorithms. The developed program was published under free GPL 3.0.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Imuno-Histoquímica
/
Antígenos Nucleares
/
Matriz Extracelular
Limite:
Humans
Idioma:
Ru
Revista:
Arkh Patol
Ano de publicação:
2017
Tipo de documento:
Article