Generation of three miR-122 knockout lines from a human embryonic stem cell line.
Stem Cell Res
; 24: 164-168, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-29034887
ABSTRACT
miR-122 is the most abundant miRNA in the human liver, accounting for 52% of the entire hepatic miRNome. Previous studies have demonstrated that miR-122 plays key roles in hepatocyte growth, metabolism, and homeostasis. Here, we created three miR-122 knockout human embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, and WAe001-A-9, using the CRISPR/Cas9 technique. These mutated cell lines retained their pluripotency, in vitro differentiation potential, normal morphology, and karyotype.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2017
Tipo de documento:
Article