Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques.
Methods Mol Biol
; 1691: 33-42, 2018.
Article
em En
| MEDLINE
| ID: mdl-29043668
ABSTRACT
The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM) which preserve the fragile structure and allow the detailed imaging of the SER.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia Eletrônica de Transmissão
/
Retículo Endoplasmático
/
Imagem Molecular
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2018
Tipo de documento:
Article