Digitonin-solubilized histamine H1-receptors bind to polyethylenimine-treated glass-fibre filters.
J Pharm Pharmacol
; 40(10): 730-3, 1988 Oct.
Article
em En
| MEDLINE
| ID: mdl-2907544
ABSTRACT
The binding of [3H]mepyramine to histamine H1-receptors solublized from guinea-pig cerebellum by 1% digitonin could be assayed by adsorption of the receptor-bound [3H]ligand to glass-fibre filters pretreated with 0.3% polyethylenimine (PEI). Non-specific binding was higher than in parallel experiments in which the bound and free [3H]ligand was separated by gel-filtration on Sephadex G-25 columns, but the parameters characterizing the inhibition curves were otherwise similar. The PEI-treated filter method could also be used to assay [3H]-(+)-N-methyl-4-methyl-diphenhydramine ([3H]QMDP) binding to solubilized H1-receptors, but the level of non-specific binding was higher and was only satisfactorily defined by icotidine or temelastine. A particular utility of the PEI-treated filter assay will be in measurements of the kinetics of H1-receptor-ligand interactions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Histamínicos H1
/
Digitonina
Limite:
Animals
Idioma:
En
Revista:
J Pharm Pharmacol
Ano de publicação:
1988
Tipo de documento:
Article