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Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method.
Maksic, Jelena; Stajic, Ana; Knezevic, Miroslav; Dacic Krnjaja, Bojana; Jancic-Stojanovic, Biljana; Medenica, Mirjana.
Afiliação
  • Maksic J; Department of Drug Control & Examination, Sector for Pharmacy, Military Medical Academy, Crnotravska 17, Belgrade, Serbia.
  • Stajic A; Department of Drug Analysis, University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia.
  • Knezevic M; University Eye Clinic, Clinical Center of Serbia, Pasterova 2, Belgrade, Serbia.
  • Dacic Krnjaja B; University Eye Clinic, Clinical Center of Serbia, Pasterova 2, Belgrade, Serbia.
  • Jancic-Stojanovic B; Department of Drug Analysis, University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia.
  • Medenica M; Department of Physical Chemistry & Instrumental Methods, University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia.
Bioanalysis ; 9(24): 1943-1954, 2017 Dec.
Article em En | MEDLINE | ID: mdl-29205055
ABSTRACT

AIM:

The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. MATERIALS &

METHODS:

Separation was performed on Acquity BEH amide column (2.1 × 100 mm, 1.7 µm). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized.

RESULTS:

The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated.

CONCLUSION:

The proposed method was utilized for monitoring of olopatadine in human tear.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lágrimas / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem / Cloridrato de Olopatadina Limite: Humans Idioma: En Revista: Bioanalysis Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lágrimas / Cromatografia Líquida de Alta Pressão / Espectrometria de Massas em Tandem / Cloridrato de Olopatadina Limite: Humans Idioma: En Revista: Bioanalysis Ano de publicação: 2017 Tipo de documento: Article