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MiR-483-5p promotes IGF-II transcription and is associated with poor prognosis of hepatocellular carcinoma.
Tang, Shaohui; Chen, Yanfang; Feng, Shufen; Yi, Tingzhuang; Liu, Xuyou; Li, Qiang; Liu, Zhilong; Zhu, Cuiping; Hu, Jianjun; Yu, Xi; Wang, Min; Cao, Guoli; Tang, Hui; Bie, Caiqun; Ma, Feng; Tang, Huijun; Du, Gang; Huang, Jianwei.
Afiliação
  • Tang S; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Chen Y; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Feng S; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Yi T; Department of Gastroenterology, Affiliated Hospital of Youjiang Medical University for Nationlities, Baise, Guangxi, China.
  • Liu X; Department of Gastroenterology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Li Q; Department of General Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Liu Z; Department of General Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Zhu C; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Hu J; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Yu X; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Wang M; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Cao G; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Tang H; Clinical Medicine Research Institute, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Bie C; Department of Gastroenterology, The Affiliated Shenzhen Shajing Hospital, Guangzhou Medical University, Shenzhen, Guangdong, China.
  • Ma F; Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Tang H; Department of Gastroenterology, The Affiliated Shenzhen Shajing Hospital, Guangzhou Medical University, Shenzhen, Guangdong, China.
  • Du G; Clinical Medicine Research Institute, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.
  • Huang J; Department of Gastroenterology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Oncotarget ; 8(59): 99871-99888, 2017 Nov 21.
Article em En | MEDLINE | ID: mdl-29245946
ABSTRACT
The human insulin-like growth factor-II (IGF-II) gene transcribes four mRNAs (P1 mRNA-P4 mRNA), and P3 mRNA overexpression contributes to hepatocarcinogenesis. IGF-II-derived miR-483-5p is implicated in the development of cancers. Here, we investigated the involvement of miR-483-5p in P3 mRNA overexpression regulation and its role in hepatocellular carcinoma. Our results showed that miR-483-5p up-regulated P3 mRNA transcription by targeting the 5'-untranslated region (5'UTR) of P3 mRNA in hepatocellular carcinoma. The mechanism was involved in recruiting of an argonaute 1(Ago1)-argonaute 2 (Ago2) complex to the P3 mRNA 5'UTR and the P3 promoter of IGF-II gene by miR-483-5p, accompanied by increased enrichment of RNA polymerase II and activating histone marks histone 3 lysine 4 trimethylation (H3K4me3), histone 3 lysine 27 acetylation (H3K27ac), and histone 4 lysine 5/8/12/16 acetylation (H4Kac) at the P3 promoter. High miR-483-5p expression was an independent predictor for shorter survival of HCC patients. The findings suggest that miR-483-5p promotes P3 mRNA transcription by recruiting the Ago1-Ago2 complex to the P3 mRNA 5'UTR and is associated with poor prognosis of HCC. Our results display a potential new model for miRNAs to up-regulate gene expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Oncotarget Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Oncotarget Ano de publicação: 2017 Tipo de documento: Article