Derivation of human iPSC line NCCSi002-A from umbilical cord blood (UCB) CD34+cells of donor from Indian ethnicity.
Stem Cell Res
; 26: 80-83, 2018 01.
Article
em En
| MEDLINE
| ID: mdl-29268156
ABSTRACT
We discuss the reprogramming of CD34+ cells isolated from UCB of a healthy female child of Indian ethnicity. The CD34+cells were nucleofected using episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). The colonies were stained for alkaline phosphatase and evaluated for pluripotency marker expression by PCR, immunofluorescence and flow-cytometry. The safety of cells was confirmed by absence of plasmid in subsequent passages by PCR. G-banded karyotype demonstrated a stable genome. The ability of tri-lineage differentiation was confirmed by specific marker expression by immunofluorescence invitro and teratoma formation invivo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Antígenos CD34
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Reprogramação Celular
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Células-Tronco Pluripotentes Induzidas
/
Sangue Fetal
Aspecto:
Determinantes_sociais_saude
Limite:
Female
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2018
Tipo de documento:
Article