Virus-like particles vaccine containing Clonorchis sinensis tegumental protein induces partial protection against Clonorchis sinensis infection.
Parasit Vectors
; 10(1): 626, 2017 12 29.
Article
em En
| MEDLINE
| ID: mdl-29284528
ABSTRACT
BACKGROUND:
Human clonorchiasis, caused by the infection of Clonorchis sinensis, is one of the major health problems in Southeast Asia. However, vaccine efficacy against C. sinensis infection remains largely unknown.METHODS:
In this study, for the first time, we generated virus-like particles (VLPs) vaccine containing the C. sinensis tegumental protein 22.3 kDa (CsTP 22.3) and the influenza matrix protein (M1) as a core protein, and investigated the vaccine efficacy in Sprague-Dawley rats.RESULTS:
Intranasal immunization of VLPs vaccine induced C. sinensis-specific IgG, IgG2a and IgG2c in the sera and IgA responses in the feces and intestines. Notably, upon challenge infection with C. sinensis metacercariae, significantly lower adult worm loads (70.2%) were measured in the liver of rats immunized with VLPs, compared to those of naïve rats. Furthermore, VLPs immunization induced antibody secreting cells (ASC) responses and CD4+/CD8+ T cell responses in the spleen.CONCLUSIONS:
Our results indicated that VLPs vaccine containing C. sinensis CsTP 22.3 kDa provided partial protection against C. sisnensis infection. Thus, VLPs could be a potential vaccine candidate against C. sinensis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-Helmínticos
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Portadores de Fármacos
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Proteínas da Matriz Viral
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Clonorquíase
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Clonorchis sinensis
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Vacinas de Partículas Semelhantes a Vírus
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Antígenos de Helmintos
Limite:
Animals
Idioma:
En
Revista:
Parasit Vectors
Ano de publicação:
2017
Tipo de documento:
Article