Ratiometric sweat secretion optical test in cystic fibrosis, carriers and healthy subjects.
J Cyst Fibros
; 17(2): 186-189, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-29292091
ABSTRACT
We have simplified the published procedure (5) for measuring sweat rates in individual human sweat glands. Sweat secretion rates were obtained from sweat drops secreted on the forearm by multiple individual glands. We computed a ratio between CFTR-dependent (by intradermal microinjection of a ß adrenergic cocktail) and CFTR-independent (by methacoline as cholinergic stimulus) sweat secretion rates. We obtained a reproducible, approximately linear readout of CFTR function with measurements performed by two different independent teams. We considered three groups (CF subjects, CF carriers and non-CF controls, n=22 in each group); their mean ratios was respectively 0.000, 0.104 and 0.205 The average ratio of CF subjects was consistent with diagnosis in 3 additional cases clinically resembling CF. All groups were clearly discriminated, with sensibility and specificity ranging from 82% to 100%. A software was developed for detecting sweat droplets. This bioassay is suitabile for multicentre studies focusing on CFTR targeted therapies, controversial diagnosis and functional relevance of rare CFTR mutations.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândulas Sudoríparas
/
Sudorese
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Fibrose Cística
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Imagem Óptica
Tipo de estudo:
Diagnostic_studies
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Observational_studies
Limite:
Humans
Idioma:
En
Revista:
J Cyst Fibros
Ano de publicação:
2018
Tipo de documento:
Article