Establishment of reverse transcription polymerase chain reaction assay for simultaneous detection of human parainfluenza virus types 1 to 4 in children with influenza like illnesses.
Bratisl Lek Listy
; 118(12): 772-776, 2017.
Article
em En
| MEDLINE
| ID: mdl-29322811
ABSTRACT
OBJECTIVES:
The aim of this study was to develop an in-house multiplex reverse transcription polymerase chain reaction (mRT-PCR), which can recognize HPIV1-4 in clinical samples.BACKGROUND:
Human parainfluenza virus (HPIV) is one of the major causes of viral respiratory infections and can affect people at any age, especially infants and young children.METHODS:
Four sets of specific primers targeting conserved areas of hemagglutinin-neuraminidase (HN) genes of HPIV1-4, were designed and tested with type-related plasmid controls. Specificity and sensitivity of mPCR were tested. One-step mRT-PCR was set up using a viral panel containing 10 respiratory viruses, including HPIVs. One hundred nasopharyngeal samples of respiratory infection patients were tested using the set One-step mRT-PCR.RESULTS:
The specificity of set mPCR for HPIV1-4 using plasmid positive controls was proved and reaction sensitivity was measured. The specificity of set mRT-PCR was confirmed and 4 and 5 out of 100 clinical samples were HPIV1 and HPIV2 positive, respectively.CONCLUSION:
The developed one-step mRT-PCR in this study is an effective and specific assay for clinical diagnosis of HPIV1 to 4 (Tab. 1, Fig. 6, Ref. 28).Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Infecções por Respirovirus
/
Infecções Respiratórias
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Paramyxoviridae
/
Infecções por Rubulavirus
Tipo de estudo:
Diagnostic_studies
Limite:
Child
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Child, preschool
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Humans
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Infant
Idioma:
En
Revista:
Bratisl Lek Listy
Ano de publicação:
2017
Tipo de documento:
Article