Ultrafast determination of vitamin E using LC-ESI-MS/MS for preclinical development of new nutraceutical formulations.
Bioanalysis
; 10(4): 215-227, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29333875
ABSTRACT
AIM:
We proposed a rapid and high quality method to determine α-tocopherol (α-T) in different biopharmaceutical samples using liquid chromatography-diode array detector on-line ESI-MS/MS. MATERIALS &METHODS:
A working standard solution of α-T and internal standard, phenyl-5,7-dimethyl-d6-α-tocopherol, were used for optimization and validation of the method. Levels of α-T in nanoemulsions, serum and plasma samples were evaluated. RESULTS &CONCLUSION:
Precision (1% for retention time, 5% for peak area and 3% for relative peak area), linearity range (among 0.625-20.0 µg ml-1), LOD and LOQ, accuracy and matrix effect were studied. The validated chromatographic method is presented as valuable analytical tool for the determination of α-tocopherol in loaded drug delivery systems and in biodistribution levels in blood samples.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vitamina E
/
Cromatografia Líquida
/
Suplementos Nutricionais
/
Espectrometria de Massas por Ionização por Electrospray
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2018
Tipo de documento:
Article