NGS-based methylation profiling differentiates TCF3-HLF and TCF3-PBX1 positive B-cell acute lymphoblastic leukemia.
Epigenomics
; 10(2): 133-147, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-29334255
ABSTRACT
AIM:
To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. MATERIALS &METHODS:
Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and after remission and analyzed using reduced representation bisulfite sequencing and RNA-sequencing.RESULTS:
We identified 7000 differentially methylated CpG sites between subtypes, of which 78% had lower methylation levels in TCF3-HLF. Gene expression was negatively correlated with CpG sites in 23 genes. KBTBD11 clearly differed in methylation and expression between subtypes and before and after remission in TCF3-HLF samples.CONCLUSION:
KBTBD11 hypomethylation may be a promising potential target for further experimental validation especially for the TCF3-HLF subtype.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia-Linfoma Linfoblástico de Células Precursoras B
/
Proteínas de Fusão Oncogênica
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Ilhas de CpG
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Metilação de DNA
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Epigênese Genética
Limite:
Adolescent
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Child
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Humans
Idioma:
En
Revista:
Epigenomics
Ano de publicação:
2018
Tipo de documento:
Article