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The extracellular matrix-degrading protein ADAMTS5 is expressed in the nuclei of urothelial cells in healthy rats.
Delbro, Dick; Hallsberg, Lena; Peeker, Ralph; Scherbak, Nikolai; Fall, Magnus; Godtman, Rebecka Arnsrud.
Afiliação
  • Delbro D; a Department of Medical Sciences , Örebro University , Örebro , Sweden.
  • Hallsberg L; b Department of Surgery , Institute of Clinical Sciences, The Sahlgrenska Academy at the University of Gothenburg , Gothenburg , Sweden.
  • Peeker R; c Department of Urology , Institute of Clinical Sciences, The Sahlgrenska Academy at the University of Gothenburg , Gothenburg , Sweden.
  • Scherbak N; d School of Science and Technology , Örebro University , Örebro , Sweden.
  • Fall M; c Department of Urology , Institute of Clinical Sciences, The Sahlgrenska Academy at the University of Gothenburg , Gothenburg , Sweden.
  • Godtman RA; c Department of Urology , Institute of Clinical Sciences, The Sahlgrenska Academy at the University of Gothenburg , Gothenburg , Sweden.
Scand J Urol ; 52(2): 139-142, 2018 Apr.
Article em En | MEDLINE | ID: mdl-29334289
OBJECTIVE: The aim of this study was to investigate whether protein expression of the extracellular matrix-degrading protease ADAMTS5 can be demonstrated in the urinary bladder of healthy rats, and, if so, to determine the localization of this enzyme. MATERIALS AND METHODS: The experiments were conducted with eight inbred male Sprague-Dawley rats. Immunohistochemistry was used to investigate the expression of ADAMTS5 in the urinary bladder. Negative controls were established by either excluding the primary antibody or applying the antibody after it had been preabsorbed with its immunogenic peptide. Confocal microscopy was used to visualize the distribution of ADAMTS5 in the urinary bladder tissue. RESULTS: Immunoreactivity for ADAMTS5 was demonstrated in the urothelium and in the detrusor. This expression was localized not only in the cytoplasm, but also in the nuclei. Confocal microscopy corroborated these findings. CONCLUSION: Expression of ADAMTS5 was demonstrated in the cytoplasm as well as in the nuclei of the urothelium and detrusor cells, suggesting that it may play a role at the transcriptional level.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bexiga Urinária / Urotélio / Proteína ADAMTS5 Limite: Animals Idioma: En Revista: Scand J Urol Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bexiga Urinária / Urotélio / Proteína ADAMTS5 Limite: Animals Idioma: En Revista: Scand J Urol Ano de publicação: 2018 Tipo de documento: Article