MicroRNA195 is associated with regulating the pathophysiologic process of human laryngeal squamous cell carcinoma.
Mol Med Rep
; 17(4): 5283-5291, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29393451
ABSTRACT
MicroRNAs (miRNAs) have been reported to be associated with the modulation of tumor development, including alterations associated with the development of human laryngeal squamous cell carcinoma (LSCC). The present study was designed to investigate whether miRNA195 was associated with the pathophysiologic process of human LSCC and to identify its potential roles and underlying molecular mechanisms. To determine whether miRNA195 serves a role in LSCC, reverse transcriptionquantitative polymerase chain reaction was used to detect miRNA195 expression in LSCC tissues. The tumorsuppressive effect of miRNA195 was determined by in vitro assays. Gainoffunction studies using miRNA195 mimics were performed to investigate cell viability, migration and invasion, and apoptosis in the AMCHN8 cell line. Western blotting was performed to reveal the molecular mechanisms of miRNA195 and its downstream signaling pathways in the LSCC AMCHN8 cell line. The present study demonstrated that miRNA195 is downregulated in primary LSCC tumors. Upregulating miRNA195 in vitro suppressed cell viability, migration and invasion in AMCHN8 cells. Overexpression of miRNA195 alone in AMCHN8 cells was sufficient to induce cell apoptosis, as identified by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Compared with the high expression of miRNA195 in AMCHN8 cells, the expression levels of vascular endothelial growth factor receptorII protein and downstream signaling pathway proteins, which were associated with cell viability, migration, invasion and apoptosis, were markedly decreased compared with control or miRNA195 negative control treatment group. Together, these data suggest the therapeutic potential of miRNA195 in modulating cell growth, migration and apoptosis during the pathophysiological progression of LSCC and that miRNA195 may serve as a potential therapeutic target in human LSCC.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Escamosas
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Regulação Neoplásica da Expressão Gênica
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Neoplasias Laríngeas
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MicroRNAs
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article