A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates.

Rampersad, Tashmin; Makume, Mantha; Sobia, Parveen; Willem Sturm, A

Rampersad, Tashmin; Makume, Mantha; Sobia, Parveen; Willem Sturm, A.

Afiliação

Rampersad T; Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
Makume M; Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
Sobia P; Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
Willem Sturm A; Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa. Electronic address: sturm@ukzn.ac.za.

J Microbiol Methods ; 146: 64-67, 2018 03.

Article em En | MEDLINE | ID: mdl-29410256

ABSTRACT

ABSTRACT

MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.

Assuntos

Antituberculosos/farmacologia; Testes Diagnósticos de Rotina/métodos; Ensaios de Triagem em Larga Escala/métodos; Testes de Sensibilidade Microbiana/métodos; Mycobacterium tuberculosis/efeitos dos fármacos; Antibacterianos/farmacologia; Técnicas Bacteriológicas/instrumentação; Técnicas Bacteriológicas/métodos; Meios de Cultura; Testes Diagnósticos de Rotina/instrumentação; Ensaios de Triagem em Larga Escala/instrumentação; Humanos; Testes de Sensibilidade Microbiana/instrumentação; Mycobacterium tuberculosis/isolamento & purificação; Reprodutibilidade dos Testes; Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico; Tuberculose Resistente a Múltiplos Medicamentos/microbiologia

Palavras-chave

Agar-dilution; Drug susceptibility test; MIC; Multi-point inoculation; Mycobacterium tuberculosis

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Testes de Sensibilidade Microbiana / Testes Diagnósticos de Rotina / Ensaios de Triagem em Larga Escala / Mycobacterium tuberculosis / Antituberculosos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google