Bridging the gap: Super-resolution microscopy of epithelial cell junctions.
Tissue Barriers
; 6(1): e1404189, 2018 01 02.
Article
em En
| MEDLINE
| ID: mdl-29420122
ABSTRACT
Cell junctions are critical for cell adhesion and communication in epithelial tissues. It is evident that the cellular distribution, size, and architecture of cell junctions play a vital role in regulating function. These details of junction architecture have been challenging to elucidate in part due to the complexity and size of cell junctions. A major challenge in understanding these features is attaining high resolution spatial information with molecular specificity. Fluorescence microscopy allows localization of specific proteins to junctions, but with a resolution on the same scale as junction size, rendering internal protein organization unobtainable. Super-resolution microscopy provides a bridge between fluorescence microscopy and nanoscale approaches, utilizing fluorescent tags to reveal protein organization below the resolution limit. Here we provide a brief introduction to super-resolution microscopy and discuss novel findings into the organization, structure and function of epithelial cell junctions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Junções Íntimas
/
Junções Aderentes
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Células Epiteliais
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Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Revista:
Tissue Barriers
Ano de publicação:
2018
Tipo de documento:
Article