Inhibition of mammalian target of rapamycin complex 1 signaling by n-3 polyunsaturated fatty acids promotes locomotor recovery after spinal cord injury.
Mol Med Rep
; 17(4): 5894-5902, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29436695
The present study aimed to explore the effects of n3 polyunsaturated fatty acids (PUFAs) on autophagy and their potential for promoting locomotor recovery after spinal cord injury (SCI). Primary neurons were isolated and cultured. SpragueDawley rats were randomly divided into three groups and fed diets with different amounts of n3 PUFAs. A model of spinal cord contusion was created at the T10 spinal segment and the composition of PUFAs was analyzed using gas chromatography. Spinal repair and motor function were evaluated postoperatively. Assessment of the effects of n3 PUFAs on autophagy and mammalian target of rapamycin complex 1 (mTORC1) was performed using immunofluorescence staining and western blotting. In vitro, n3 PUFAs inhibited mTORC1 and enhanced autophagy. The n3 PUFA levels and the ratio of n3 PUFA to n6 PUFA in the spinal cord and serum of rats fed a highn3 PUFA diet were higher before and after operation (P<0.05). Additionally, rats in the highn3 PUFA group showed improved motor function recovery, spinal cord repairrelated protein expression level (MBP, Galc and GFAP). Expression levels if these protiens in the highn3 PUFA diet group expressed the highest levels, followed by the lown3 PUFA diet group and finally the control group (P<0.05). highn3 PUFA diet promoted autophagy ability and inhibited activity of the mTORC1 signaling pathway compared with the lown3 PUFA diet group or the control group (P<0.05). These results suggest that exogenous dietary n3 PUFAs can inhibit mTORC1 signaling and enhance autophagy, promoting functional recovery of rats with SCI.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismos da Medula Espinal
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Transdução de Sinais
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Ácidos Graxos Ômega-3
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Recuperação de Função Fisiológica
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Alvo Mecanístico do Complexo 1 de Rapamicina
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Atividade Motora
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article