Epididymal sperm from Spix's yellow-toothed cavies sperm successfully cryopreserved in Tris extender with 6% glycerol and 20% egg yolk.
Anim Reprod Sci
; 191: 64-69, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-29463464
ABSTRACT
As a non-threatened hystricognath rodent species, Spix's yellow-toothed cavies can be used as a model for the development of assisted reproductive techniques for the conservation of closely related species. The objective was to establish a functional protocol for cryopreservation of epididymal sperm from these cavies. Twelve sexually mature males, â¼2â¯y old and weighing â¼300â¯g, were euthanized. Sperm were recovered by retrograde flushing of the vas deferens and cauda epididymis with Tris extender. Thereafter, sperm were extended in Tris plus 20% egg yolk, with 3%, 6% or 9% glycerol or dimethyl sulfoxide (DMSO), placed in 0.25â¯mL straws and cryopreserved in liquid nitrogen. Sperm concentration, motility (using computer-assisted sperm analysis; CASA), plasma membrane integrity, osmotic response, morphology and sperm binding-ability were determined in fresh and frozen-thawed sperm. For most sperm endpoints, glycerol was a more desirable cryoprotectant than DMSO. Data (mean⯱â¯SEM) were similar with use of 3%, 6%, and 9% glycerol (Pâ¯>â¯0.05) in osmotic response (40.66⯱â¯6.3%, 42.5⯱â¯7.1%, and 39.5⯱â¯5.0% respectably), and membrane integrity (55.17⯱â¯5.5%, 68.4⯱â¯4.1%, and 59.1⯱â¯4.9% respectably). Among concentrations assessed, the use of 6% glycerol resulted in the greatest (Pâ¯<â¯0.05) post-thaw values for total motility (60.9⯱â¯4.4%), rapid subpopulation motility (27.7⯱â¯3.1%) and sperm-binding capability (227.0⯱â¯20.2). In conclusion, epididymal sperm from the Spix's yellow-toothed cavies (G. spixii) are optimally cryopreserved in Tris extender with 6% glycerol and 20% egg yolk.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
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Criopreservação
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Crioprotetores
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Gema de Ovo
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Análise do Sêmen
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Glicerol
Limite:
Animals
Idioma:
En
Revista:
Anim Reprod Sci
Ano de publicação:
2018
Tipo de documento:
Article