Copper nanoclusters as probes for turn-on fluorescence sensing of L-lysine.
Talanta
; 182: 595-599, 2018 May 15.
Article
em En
| MEDLINE
| ID: mdl-29501198
ABSTRACT
Herein, a unique protocol based on copper nanoclusters (CuNCs) probe for turn-on fluorescence sensing of L-lysine was developed. The fluorescent CuNCs with ovalbumin as the stabilizer was prepared by a simple, one-step and green method. When 370â¯nm was used as the excitation wavelength, the resultant CuNCs exhibited a pale blue fluorescence with the maximum emission at 440â¯nm. Interestingly, existence of L-lysine evoked the obvious fluorescence intensity increase of CuNCs. The detection limit of the proposed method for L-lysine was 5.5⯵M, with a good linear range from 10.0⯵M to 1.0â¯mM (r2 =â¯0.999). Moreover, the possible mechanism for enhanced fluorescence intensity of CuNCs by addition of L-lysine was explored and discussed briefly. Further, the as-prepared fluorescent CuNCs was successfully applied in detection of L-lysine in urine. Our results demonstrated that L-lysine could be monitored by the probe, providing new path for construction of CuNCs as fluorescent probes and showing great potential in quantification of L-lysine in real samples.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
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Cobre
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Nanoestruturas
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Nanopartículas Metálicas
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Corantes Fluorescentes
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Lisina
Limite:
Humans
Idioma:
En
Revista:
Talanta
Ano de publicação:
2018
Tipo de documento:
Article