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Comparative Characterization of Four Calcium-Binding EF Hand Proteins from Opisthorchis viverrini.
Emmanoch, Palida; Kosa, Nanthawat; Vichasri-Grams, Suksiri; Tesana, Smarn; Grams, Rudi; Geadkaew-Krenc, Amornrat.
Afiliação
  • Emmanoch P; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12120, Thailand.
  • Kosa N; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12120, Thailand.
  • Vichasri-Grams S; Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
  • Tesana S; Food-borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen, 40002, Thailand.
  • Grams R; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12120, Thailand.
  • Geadkaew-Krenc A; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12120, Thailand.
Korean J Parasitol ; 56(1): 81-86, 2018 Feb.
Article em En | MEDLINE | ID: mdl-29529855
Four isoforms of calcium binding proteins containing 2 EF hand motifs and a dynein light chain-like domain in the human liver fluke Opisthorchis viverrini, namely OvCaBP1, 2, 3, and 4, were characterized. They had molecular weights of 22.7, 21.6, 23.7, and 22.5 kDa, respectively and showed 37.2-42.1% sequence identity to CaBP22.8 of O. viverrini. All were detected in 2- and 4-week-old immature and mature parasites. Additionally, OvCaBP4 was found in newly excysted juveniles. Polyclonal antibodies against each isoform were generated to detect the native proteins in parasite extracts by Western blot analysis. All OvCaBPs were detected in soluble and insoluble crude worm extracts and in the excretory-secretory product, at approximate sizes of 21-23 kDa. The ion-binding properties of the proteins were analyzed by mobility shift assays with the divalent cations Ca2+, Mg2+, Zn2+, and Cu2+. All OvCaBPs showed mobility shifts with Ca2+ and Zn2+. OvCaBP1 showed also positive results with Mg2+ and Cu2+. As tegumental proteins, OvCaBP1, 2, and 3 are interesting drug targets for the treatment of opisthorchiasis.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Opistorquíase / Opisthorchis / Proteínas de Ligação ao Cálcio / Proteínas de Helminto / Motivos EF Hand Limite: Animals Idioma: En Revista: Korean J Parasitol Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Opistorquíase / Opisthorchis / Proteínas de Ligação ao Cálcio / Proteínas de Helminto / Motivos EF Hand Limite: Animals Idioma: En Revista: Korean J Parasitol Ano de publicação: 2018 Tipo de documento: Article