Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.
J Eukaryot Microbiol
; 65(5): 722-728, 2018 07.
Article
em En
| MEDLINE
| ID: mdl-29542839
ABSTRACT
Membrane proteins in trypanosomatids are, in general, weakly expressed and confirmation of their subcellular localization frequently requires their overexpression with epitope tags. However, overexpression can lead to mislocalization of the probes. Viswanathan et al. (Nat. Methods, 2015, 12568) described high performance tags for localization of weakly expressed proteins. We report here the use of these protein tags, named "spaghetti monster," for CRISPR/Cas9-mediated C-terminal endogenous tagging of Trypanosoma cruzi to localize two weakly expressed transient receptor potential channels to acidic compartments. The results indicate that this method will improve the detection of membrane proteins in T. cruzi.
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Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Proteínas de Protozoários
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Eukaryot Microbiol
Ano de publicação:
2018
Tipo de documento:
Article