Your browser doesn't support javascript.
loading
Alternative RNA splicing of leucocyte tissue transglutaminase in coeliac disease.
Arbildi, P; Sóñora, C; Del Río, N; Marqués, J M; Hernández, A.
Afiliação
  • Arbildi P; Cátedra de Inmunología, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
  • Sóñora C; Cátedra de Inmunología, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
  • Del Río N; Facultad de Medicina, Escuela Universitaria de Tecnología Médica, Universidad de la República, Montevideo, Uruguay.
  • Marqués JM; Cátedra de Inmunología, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
  • Hernández A; Departamento de Desarrollo Biotecnológico, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Montevideo, Uruguay.
Scand J Immunol ; 87(5): e12659, 2018 May.
Article em En | MEDLINE | ID: mdl-29543397
Tissue transglutaminase is a ubiquitous and multifunctional protein that contributes to several processes such as apoptosis/survival, efferocytosis, inflammation and tissue repairing under physiological and pathological conditions. Several activities can be associated with well-established functional domains; in addition, four RNA alternative splice variants have been described, characterized by sequence divergences and residues deletion at the C-terminal domains. Tissue transglutaminase is recognized as the central player in the physiopathology of coeliac disease (CD) mainly through calcium-dependent enzymatic activities. It can be hypothesized that differential regulation of tissue transglutaminase splice variants expression in persons with CD contributes to pathology by altering the protein functionality. We characterized the expression pattern of RNA alternative splice variants by RT-PCR in peripheral cells from patients with CD under free gluten diet adhesion; we considered inflammatory parameters and specific antibodies as markers of the stage of disease. We found significant higher expression of both the full length and the shortest C-truncated splice variants in leucocytes from patients with CD in comparison with healthy individuals. As tissue transglutaminase expression and canonical enzymatic activity are linked to inflammation, we studied the RNA expression of inflammatory cytokines in peripheral leucocytes of persons with CD in relation with splice variants expression; interestingly, we found that recently diagnosed patients showed significant correlation between both the full length and the shortest alternative spliced variants with IL-1 expression. Our results points that regulation of alternative splicing of tissue transglutaminase could account for the complex physiopathology of CD.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Celíaca / Transglutaminases / Processamento Alternativo / Proteínas de Ligação ao GTP / Leucócitos Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Scand J Immunol Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença Celíaca / Transglutaminases / Processamento Alternativo / Proteínas de Ligação ao GTP / Leucócitos Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Scand J Immunol Ano de publicação: 2018 Tipo de documento: Article