A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses.
Nat Commun
; 9(1): 1230, 2018 03 26.
Article
em En
| MEDLINE
| ID: mdl-29581442
ABSTRACT
Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The "Sementis Copenhagen Vector" (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination of mice induces neutralizing antibodies to both viruses in wild-type and IFNAR-/- mice and protects against (i) chikungunya virus viremia and arthritis in wild-type mice, (ii) Zika virus viremia and fetal/placental infection in female IFNAR-/- mice, and (iii) Zika virus viremia and testes infection and pathology in male IFNAR-/- mice. To our knowledge this represents the first single-vector construct, multi-pathogen vaccine encoding large polyproteins, and offers both simplified manufacturing and formulation, and reduced "shot burden" for these often co-circulating arboviruses.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
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2_ODS3
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3_ND
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4_TD
Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
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Vacinas Virais
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Vírus Chikungunya
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Febre de Chikungunya
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Zika virus
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Infecção por Zika virus
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Vetores Genéticos
Limite:
Animals
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Female
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Humans
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Male
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Pregnancy
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2018
Tipo de documento:
Article