Ultrasound assisted enzymatic hydrolysis for isolating titanium dioxide nanoparticles from bivalve mollusk before sp-ICP-MS.
Anal Chim Acta
; 1018: 16-25, 2018 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-29605130
ABSTRACT
Applicability of single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) using dwell times equal to or shorter than 100⯵s has been tested for assessing titanium dioxide nanoparticles (TiO2 NPs) in bivalve mollusks. TiO2 NPs isolation from fresh mollusk tissues was achieved by ultrasound assisted enzymatic hydrolysis procedure using a pancreatin/lipase mixture. Optimum extraction conditions imply ultrasonication (60% amplitude) for 10â¯min, and 7.5â¯mL of a solution containing 3.0â¯g L-1of pancreatin and lipase (pH 7.4). The developed method was found to be repeatable (repeatability of 17% for the over-all procedure, TiO2 NPs concentration of 5.33â¯×â¯107⯱â¯8.89â¯×â¯106, nâ¯=â¯11), showing a limit of detection of 5.28â¯×â¯106 NPs g-1, and a limit of detection in size of 24.4-30.4â¯nm, based on the 3σ criteria, and on the 3σ/5 σ criteria, respectively. The analytical recovery within the 90-99% range (use of TiO2 NPs standards of 50â¯nmâ¯at 7 and 14⯵gâ¯L-1 as Ti). Several bivalve mollusks (clams, cockles, mussels, razor clams, oysters and variegated scallops) were analyzed for total titanium (ICP-MS after microwave assisted acid digestion), and for TiO2 NPs by the proposed method. TiO2 NPs concentrations were within the 2.36â¯×â¯107-1.25â¯×â¯108 NPs g-1 range, and the most frequent sizes were from 50 to 70â¯nm.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Titânio
/
Pancreatina
/
Bivalves
/
Nanopartículas
/
Ondas Ultrassônicas
/
Lipase
Limite:
Animals
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2018
Tipo de documento:
Article