A comprehensive web tool for toehold switch design.
Bioinformatics
; 34(16): 2862-2864, 2018 08 15.
Article
em En
| MEDLINE
| ID: mdl-29648573
ABSTRACT
Motivation Toehold switches are a class of RNAs with a hairpin loop that can be unfolded upon binding a trigger RNA, thereby exposing a ribosome binding site (RBS) and permitting translation of the reporter protein. They have been shown very useful in detecting a variety of targets including RNAs from Zika and Ebola viruses. The base complementation between the toehold switch and the trigger RNA also makes it sensitive to sequence variations. Design of toehold switches involves a series of considerations related to their sequence properties, structures and specificities. Results:
Here we present the first comprehensive web tool for designing toehold switches. We also propose a score for predicting the efficacy of designed toehold switches based on properties learned from â¼180 experimentally tested switches. Availability and implementation The toehold switch web tool is available at https//yiplab.cse.cuhk.edu.hk/toehold/.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Design de Software
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Bioinformatics
Ano de publicação:
2018
Tipo de documento:
Article