Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells.
Methods Mol Biol
; 1773: 231-259, 2018.
Article
em En
| MEDLINE
| ID: mdl-29687394
ABSTRACT
The development of simple but effective storage protocols for adult stem cells will greatly enhance their use and utility in tissue-engineering applications. Cryopreservation has shown to be most promising but is a fairly complex process, necessitating the use of chemicals called cryoprotective agents (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Tecido Adiposo
/
Crioprotetores
/
Células-Tronco Mesenquimais
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2018
Tipo de documento:
Article